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英语翻译CDR-H3residesinthecenteroftheP5a/P5chelicalinterfaceatthethree-helixjunction(Fig.5A).ResiduesGly-98,Ser-100,andThr100ainteractwiththewideandshallowminorgrooveofP5cviadirectHbonds,andTyr-102contactsP5abyHbon
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更新时间:2024-03-28 20:11:26
问题描述:

英语翻译

CDR-H3residesinthecenteroftheP5a/P5chelicalinterfaceat

thethree-helixjunction(Fig.5A).ResiduesGly-98,Ser-100,and

Thr100ainteractwiththewideandshallowminorgrooveofP5cvia

directHbonds,andTyr-102contactsP5abyHbondingtoU131in

theminorgroove.SeveralCDR-H3residuesformapocket(Fig.

5B)thatenclosesU130,thebulgeduridineinP5a,andtheyusea

varietyofmolecularstrategiestointeractwithU130.Arg-94,

Ala-96,Asp-101,andGly100cformdirectHbondstotheuracil

baseandsugarring,whereasthesidechainsofAla-96andMet-99

embracethenucleotidewithnonpolarcontacts.Tyr100bstacks

perpendicularlywiththebase,andArg-94formsanN-Obridgetype

ofionpairwiththeadjacentphosphate(36).Interestingly,

althoughArg-95wasselectedfromthelibrary,itmakesnointeraction

withtheRNAinthestructure;instead,itssidechainpoints

awayfromtheRNAandmakeshydrogenbondinginteractionswith

thebackbonecarbonylgroupsofTyr100bandMet-99,possibly

stabilizingtheCDR-H3loopconformation.

CDR-L2loopresidues(definedasresidues50–56)(37)andthe

flankingframeworkresiduesinteractextensivelywithRNA,mainly

intheP5aminorgroove.ResiduesSer-56,Gly-57,andSer-60form

directHbondstothe32strandofP5a,acrossfromU130nearthe

hingeregion(Fig.5A).Apossiblelong-rangeionpairbetween

Arg-61andthephosphatemoietyofC197maystabilizethis

interaction.DirectHbondingbetweenSer-50andtheU167

phosphatelinksCDR-L2toP5c,makingL2anotherCDRthat

bindsbothhelicalstems.Tyr-49andTyr-55stackperpendicularly

withU130fromthenonpolarsidetosealtheU130-bindingpocket

generatedbyCDR-H3(Fig.5B).Threewatermoleculesorganized

bythefourtyrosineresiduessurroundingU130interactwiththe

sugar–phosphatemoiety,whichmayfurtherstrengthentheRNAFab

interactionatthenonpolarinterfaceoftheU130binding

pocket(Fig.5C).

我需要大致了解

陈文喻回答:
  CDR-H3居住在中心的P5a/P5c螺旋接口three-helix交界处的(图5).残留Gly-98,萨尔-100,Thr100a交互槽宽,shallowminorP5c通过直接H债券,和接触P5a钟-102通过催眠与U131小槽.几个CDR-H3残留(图形成一个口袋里.5B)...
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